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h7 hescs  (WiCell Research Institute Inc)


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    Structured Review

    WiCell Research Institute Inc h7 hescs
    H7 Hescs, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h7+hescs/WA07/bio_rxiv__2025__11__11__687613-39-1-5
    Average 96 stars, based on 183 article reviews
    h7 hescs - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Suspension:

    Article Title: SARS-CoV-2 infects human pluripotent stem cell-derived cardiomyocytes, impairing electrical and mechanical function
    Article Snippet: Heat-shock was performed on day 13 for 30 min at 42 °C, and on day 14 cardiomyocytes were dissociated and frozen in CS10 cryopreservation media (Sigma-Aldrich). .. H7 hESCs (WA07; WiCell) were differentiated in suspension culture format by collaborators at the Center for Applied Technology Development at the City of Hope in California (as previously described ), received by dry-ice shipment, and stored in liquid nitrogen before use. ..

    Article Title: SARS-CoV-2 Infects Human Pluripotent Stem Cell-Derived Cardiomyocytes, Impairing Electrical and Mechanical Function
    Article Snippet: Heat-shock was performed on day 13 for 30 min at 42 °C, and on day 14 cardiomyocytes were dissociated and frozen in CS10 cryopreservation media (Sigma-Aldrich). .. H7 hESCs (WA07; WiCell) were differentiated in suspension culture format by collaborators at the Center for Applied Technology Development at the City of Hope in California (as previously described (Chen et al., 2015), received by dry-ice shipment, and stored in liquid nitrogen before use. .. Undifferentiated H9 hESCs (WA09; WiCell) were maintained and differentiated into LM-SMCs and NC-SMCs as previously described (Bargehr et al., 2016; Serrano et al., 2019).

    Article Title: SARS-CoV-2 Infects Human Pluripotent Stem Cell-Derived Cardiomyocytes, Impairing Electrical and Mechanical Function
    Article Snippet: Heat-shock was performed on day 13 for 30 min at 42 °C, and on day 14 cardiomyocytes were dissociated and frozen in CS10 cryopreservation media (Sigma-Aldrich). .. H7 hESCs (WA07; WiCell) were differentiated in suspension culture format by collaborators at the Center for Applied Technology Development at the City of Hope in California (as previously described (Chen et al., 2015), received by dry-ice shipment, and stored in liquid nitrogen before use. .. Undifferentiated H9 hESCs (WA09; WiCell) were maintained and differentiated into LM-SMCs and NC-SMCs as previously described (Bargehr et al., 2016; Serrano et al., 2019).

    other:


    FACS:

    Article Title: Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants
    Article Snippet: Two guide RNAs targeting exon 22 (Supplementary Fig. ) of the human DLG2 gene, covering the first PDZ domain, were designed using a web-based tool (crispr.mit.edu) and cloned into two plasmids containing D10A nickase mutant Cas9 with GFP (PX461) or Puromycin resistant gene (PX462) . pSpCas9n(BB)-2A-GFP (PX461) and pSpCas9n(BB)-2A-Puro (PX462) was a gift from Feng Zhang (For PX461, Addgene plasmid#48140; http://n2t.net/addgene:48140 ; RRID:Addgene_48140; For PX462, Addgene plasmid #48141; http://n2t.net/addgene:48141 ; RRID:Addgene_48141). .. H7 hESCs (WiCell) were nucleofected using P4 solution and CB150 programme (Lonza) with 5 μg of plasmids, FACS sorted on the following day and plated at a low density (~70 cells/cm 2 ) for clonal isolation. ..

    Isolation:

    Article Title: Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants
    Article Snippet: Two guide RNAs targeting exon 22 (Supplementary Fig. ) of the human DLG2 gene, covering the first PDZ domain, were designed using a web-based tool (crispr.mit.edu) and cloned into two plasmids containing D10A nickase mutant Cas9 with GFP (PX461) or Puromycin resistant gene (PX462) . pSpCas9n(BB)-2A-GFP (PX461) and pSpCas9n(BB)-2A-Puro (PX462) was a gift from Feng Zhang (For PX461, Addgene plasmid#48140; http://n2t.net/addgene:48140 ; RRID:Addgene_48140; For PX462, Addgene plasmid #48141; http://n2t.net/addgene:48141 ; RRID:Addgene_48141). .. H7 hESCs (WiCell) were nucleofected using P4 solution and CB150 programme (Lonza) with 5 μg of plasmids, FACS sorted on the following day and plated at a low density (~70 cells/cm 2 ) for clonal isolation. ..



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